Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-Adenosine (Bz) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

제품 번호CH65017CAS 번호110782-31-5순도100.0% (NMR)
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제품 개요

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제품군
Oligonucleotide Synthesis, RNA Raw Materials & Modification
분자식
C48H54N7O8P
분자량
888.0
순도
100.0% (NMR)

2'-OMe-Adenosine (Bz) CE Phosphoramidite is the 2'-O-methyl adenosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N6-benzoyl-2'-O-methyladenosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65017 is identified by CAS 110782-31-5, molecular formula C48H54N7O8P, molecular weight 888.0, PubChem CID 13872235, and InChIKey AZCGOTUYEPXHMJ-PSVHYZMASA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

동의어

DMT-2'-OMe-rA(Bz) phosphoramidite; 5'-O-DMT-N6-benzoyl-2'-O-methyladenosine 3'-CE phosphoramidite

식별 정보 및 규격

Catalog No.CH65017
CAS No.110782-31-5
Molecular FormulaC48H54N7O8P
Molecular Weight888.0
PubChem CID13872235
InChIKeyAZCGOTUYEPXHMJ-PSVHYZMASA-N
Purity100.0% (NMR)

보관 조건

-20 C

기술 속성

Monomer class
2'-O-methyl RNA CE phosphoramidite
Nucleobase
Adenine
Sugar modification
2'-O-Methyl
Base protection
N6-Benzoyl
Grade
Standard; TheraPure
Packaging
250 mg; 1 g; 5 g; 10 g; 500 g; Custom packaging on request
Water content
<=0.2% by Karl Fischer
Physical form
white powder

응용 맥락

  • 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
  • 2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
  • 5'-DMT + N6-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection.

관련 기술 자료

공개 출처

자주 묻는 질문

How does it relate to 5'-O-DMT-N6-benzoyl-2'-O-methyladenosine?

It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.

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