2'-OMe-Uridine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
제품 개요
빠른 보기
- 제품군
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 분자식
- C40H49N4O9P
- 분자량
- 760.8
- 순도
- 100.0% (NMR)
2'-OMe-Uridine CE Phosphoramidite is the 2'-O-methyl uridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-methyluridine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65020 is identified by CAS 110764-79-9, molecular formula C40H49N4O9P, molecular weight 760.8, PubChem CID 10897989, and InChIKey UVUOJOLPNDCIHL-XKZJCBTISA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because uracil has no exocyclic amine, no base protecting group is required. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
동의어
DMT-2'-OMe-rU phosphoramidite; 5'-O-DMT-2'-O-methyluridine 3'-CE phosphoramidite
식별 정보 및 규격
| Catalog No. | CH65020 |
| CAS No. | 110764-79-9 |
| Molecular Formula | C40H49N4O9P |
| Molecular Weight | 760.8 |
| PubChem CID | 10897989 |
| InChIKey | UVUOJOLPNDCIHL-XKZJCBTISA-N |
| Purity | 100.0% (NMR) |
보관 조건
-20 C
기술 속성
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Uracil
- Sugar modification
- 2'-O-Methyl
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
응용 맥락
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- No base protection needed: uracil has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
관련 기술 자료
공개 출처
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 화합물 10897989
PubChem · CID 10897989
자주 묻는 질문
Why does the 2'-OMe-uridine phosphoramidite have no base protecting group?
Uracil has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-O-methyluridine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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