2'-F-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
제품 개요
빠른 보기
- 제품군
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 분자식
- C44H53FN7O8P
- 분자량
- 857.9
- 순도
- 99.9% (HPLC)
2'-F-Guanosine (iBu) CE Phosphoramidite is the 2'-fluoro guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65028 is identified by CAS 144089-97-4, molecular formula C44H53FN7O8P, molecular weight 857.9, PubChem CID 135448972, and InChIKey KJFUMXZZVYMQEU-AOCJBPQJSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
동의어
DMT-2'-F-rG(iBu) phosphoramidite; 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine 3'-CE phosphoramidite
식별 정보 및 규격
| Catalog No. | CH65028 |
| CAS No. | 144089-97-4 |
| Molecular Formula | C44H53FN7O8P |
| Molecular Weight | 857.9 |
| PubChem CID | 135448972 |
| InChIKey | KJFUMXZZVYMQEU-AOCJBPQJSA-N |
| Purity | 99.9% (HPLC) |
보관 조건
-20 C
기술 속성
- Monomer class
- 2'-fluoro RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N2-Isobutyryl
- Packaging
- 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.3%
- Physical form
- white to off-white powder
- Stability
- stable under recommended storage conditions
응용 맥락
- 2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
관련 기술 자료
공개 출처
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 화합물 135448972
PubChem · CID 135448972
자주 묻는 질문
What does this 2'-F amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-isobutyryl removed at final deprotection. The product provides a 2'-fluoro guanosine phosphoramidite identity.
How does it relate to 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
유사 제품
소재 선택에 도움이 필요하신가요?
CHEMOS는 제품 적합성, 목표 구조, 경로 이슈, 분석 기대치 및 프로젝트별 공급 요구를 검토합니다.