Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-Deoxyinosine

9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one

製品番号CH64006CAS番号890-38-0純度>98.0% (HPLC, titration)

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製品概要

クイックビュー

製品ファミリー: Oligonucleotide Synthesis, RNA Raw Materials & Modification
化学名: 9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one
分子式: C10H12N4O4
分子量: 252.23
純度: >98.0% (HPLC, titration)

2'-Deoxyinosine (dI) is the purine DNA nucleoside built from hypoxanthine and 2-deoxy-β-D-ribose. CH64006 is listed under CAS 890-38-0, molecular formula C10H12N4O4, molecular weight 252.23, and PubChem CID 135398593.

This record is distinct from inosine, the corresponding RNA nucleoside. Deoxyinosine is used as a universal-base residue in degenerate primer and probe design, and incorporated hypoxanthine provides an endonuclease V recognition site for nucleic-acid amplification decontamination studies.

別名

2'-Deoxyinosine; deoxyinosine; dI; hypoxanthine 2'-deoxyriboside

同定情報と仕様

Catalog No.	CH64006
CAS No.	890-38-0
Molecular Formula	C10H12N4O4
Molecular Weight	252.23
PubChem CID	135398593
InChIKey	VGONTNSXDCQUGY-RRKCRQDMSA-N
Purity	>98.0% (HPLC, titration)

保管条件

-20 C

技術属性

Nucleobase: Hypoxanthine
Sugar: 2'-Deoxyribose
Nucleoside class: Purine 2'-deoxyribonucleoside
Packaging: 1 g glass bottle with plastic insert; Custom packaging on request
Physical form: Crystalline solid
Stability: >=4 years

用途情報

Degenerate primer and probe design: deoxyinosine pairs with all four canonical bases, and nearest-neighbor parameters are used in degenerate PCR-primer and microarray-probe design research.
Endonuclease V decontamination studies: incorporated hypoxanthine from dI is recognized by endonuclease V in carryover-amplicon cleavage methods.
dI nucleoside reference: CH64006 provides the deoxyinosine identity for nucleoside and oligonucleotide chemistry comparisons.

公開情報源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem化合物 135398593
PubChem · CID 135398593

よくある質問

Why is deoxyinosine called a universal base?

Deoxyinosine (dI) pairs with all four canonical bases (C, A, T, G) with relatively low discrimination, so it is used as a universal-base position in degenerate primers and probes.

How is deoxyinosine used in amplification decontamination?

When deoxyinosine is incorporated, the resulting hypoxanthine is the recognition site for endonuclease V, which cleaves carryover amplicons while leaving the intended template intact.