5'-O-DMT-2'-fluoro-N4-acetyl-2'-deoxycytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
製品概要
クイックビュー
- 製品ファミリー
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C32H32FN3O7
- 分子量
- 589.6
- 純度
- >=99%
5'-O-DMT-2'-fluoro-N4-acetyl-2'-deoxycytidine is 2'-fluoro-2'-deoxycytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-acetyl base-protecting group. CH64124 is identified by CAS 159414-98-9, molecular formula C32H32FN3O7, molecular weight 589.6, PubChem CID 22094553, and InChIKey JIFNUYITABZZQS-PYYPWFDZSA-N.
It is a 2'-F-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N4-acetyl group masks the cytosine exocyclic amine and is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
別名
5'-O-DMT-2'-fluoro-N4-acetyl-2'-deoxycytidine; 5'-O-DMT-2'-F-rC(Ac); DMT-2'-F-rC(Ac)
同定情報と仕様
| Catalog No. | CH64124 |
| CAS No. | 159414-98-9 |
| Molecular Formula | C32H32FN3O7 |
| Molecular Weight | 589.6 |
| PubChem CID | 22094553 |
| InChIKey | JIFNUYITABZZQS-PYYPWFDZSA-N |
| Purity | >=99% |
保管条件
2-8 C, tightly closed, protected from light and humidity
技術属性
- Nucleobase
- Cytosine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N4-Acetyl
- Grade
- N (Normal)
- Packaging
- 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 1 kg; 10 kg; 25 kg; 1 mL; Custom packaging on request
- Solution concentration
- 10 mM in DMSO
- Water content
- <=4.0%
- Physical form
- white, off-white to faint yellow powder
- Stability
- Powder: 2 years at 2-8 C; in solution: 6 months at -20 C; 1 year at -80 C
用途情報
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
関連技術資料
公開情報源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem化合物 22094553
PubChem · CID 22094553
よくある質問
How does it differ from N4-acetyl-2'-fluoro-2'-deoxycytidine?
Both carry an N4-acetyl-protected 2'-fluoro cytidine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.
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