CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65011CAS番号121058-88-6純度100% (HPLC)
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製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C47H64N5O9PSi
分子量
902.1
純度
100% (HPLC)

2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite is the fully protected cytidine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-acetylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65011 is identified by CAS 121058-88-6, molecular formula C47H64N5O9PSi, molecular weight 902.1, PubChem CID 11814945, and InChIKey QKWKXYVKGFKODW-YOEDQOPESA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, the 2'-O-TBDMS protects the ribose 2'-hydroxyl, and the N4-acetyl base group is removed at final deprotection.

別名

DMT-2'-O-TBDMS-rC(Ac) phosphoramidite; 5'-O-DMT-2'-O-TBDMS-N4-acetylcytidine 3'-CE phosphoramidite

同定情報と仕様

Catalog No.CH65011
CAS No.121058-88-6
Molecular FormulaC47H64N5O9PSi
Molecular Weight902.1
PubChem CID11814945
InChIKeyQKWKXYVKGFKODW-YOEDQOPESA-N
Purity100% (HPLC)

保管条件

-20 C

技術属性

Monomer class
RNA CE phosphoramidite
Nucleobase
Cytosine
2'-O protection
TBDMS (tert-butyldimethylsilyl)
Base protection
N4-Acetyl
Grade
N (Normal)
Packaging
250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
Water content
<=0.3% (same-SKU COA specification)
Physical form
white to light yellow powder
Stability
Manufactured 2022-09-12; retest 2026-03-15; expiry 2026-09-11

用途情報

  • RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
  • 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
  • N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What are the four functional groups on this RNA amidite?

A 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite (the reactive coupling group), an acid-labile 5'-O-DMT group (removed each cycle), a 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and an N4-acetyl base protection (removed at final deprotection).

How does it differ from the 2'-O-TBDMS-rC(Bz) phosphoramidite?

Both are 2'-O-TBDMS cytidine RNA amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.

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