CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-O-TBDMS-Uridine CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65013CAS番号118362-03-1純度100% (NMR)
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製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C45H61N4O9PSi
分子量
861.0
純度
100% (NMR)

2'-O-TBDMS-Uridine CE Phosphoramidite is the uridine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-uridine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65013 is identified by CAS 118362-03-1, molecular formula C45H61N4O9PSi, molecular weight 861.0, PubChem CID 10898199, and InChIKey SKNLXHRBXYGJOC-ZMHKPELYSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the 2'-O-TBDMS protects the ribose 2'-hydroxyl. Because uracil has no exocyclic amine, no base protecting group is required.

別名

DMT-2'-O-TBDMS-rU phosphoramidite; 5'-O-DMT-2'-O-TBDMS-uridine 3'-CE phosphoramidite

同定情報と仕様

Catalog No.CH65013
CAS No.118362-03-1
Molecular FormulaC45H61N4O9PSi
Molecular Weight861.0
PubChem CID10898199
InChIKeySKNLXHRBXYGJOC-ZMHKPELYSA-N
Purity100% (NMR)

保管条件

-20 C; dry, inert atmosphere

技術属性

Monomer class
RNA CE phosphoramidite
Nucleobase
Uracil
2'-O protection
TBDMS (tert-butyldimethylsilyl)
Base protection
Unprotected
Grade
N (Normal)
Packaging
250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
Water content
K.F. <=0.20% w/w
Physical form
white to light yellow powder
Stability
Manufactured 2023-10-24; retest 2027-04-26; expiry 2027-10-23

用途情報

  • RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
  • 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
  • No base protection needed: uracil has no exocyclic amine, so this monomer requires no base protecting group.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

Why does the uridine RNA phosphoramidite have no base protecting group?

Uracil has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group (removed each cycle), the 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and the 3'-phosphoramidite coupling group.

How does it relate to 5'-O-DMT-2'-O-TBDMS-uridine?

It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.

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