CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-O-TBDMS-Guanosine (dmf) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65015CAS番号149559-87-5純度>=98.0% (HPLC)
製品画像はありません

製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C49H67N8O8PSi
分子量
955.2
純度
>=98.0% (HPLC)

2'-O-TBDMS-Guanosine (dmf) CE Phosphoramidite is the fully protected guanosine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N2-dimethylformamidine-guanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65015 is identified by CAS 149559-87-5, molecular formula C49H67N8O8PSi, molecular weight 955.2, and supplier-resolved stereochemical InChIKey RQXRLANMGPTUJP-MVEMIKKVSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, the 2'-O-TBDMS protects the ribose 2'-hydroxyl, and the N2-dmf base group is removed at final deprotection.

別名

DMT-2'-O-TBDMS-rG(dmf) phosphoramidite; 5'-O-DMT-2'-O-TBDMS-N2-dmf-guanosine 3'-CE phosphoramidite

同定情報と仕様

Catalog No.CH65015
CAS No.149559-87-5
Molecular FormulaC49H67N8O8PSi
Molecular Weight955.2
PubChem CID169545671 (non-stereochemical cross-reference)
Stereochemical InChIKeyRQXRLANMGPTUJP-MVEMIKKVSA-N
Purity>=98.0% (HPLC)

保管条件

-20 C; dry, inert atmosphere

技術属性

Monomer class
RNA CE phosphoramidite
Nucleobase
Guanine
2'-O protection
TBDMS (tert-butyldimethylsilyl)
Base protection
N2-Dimethylaminomethylene (dmf)
Grade
N (Normal)
Packaging
100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 1 kg; 5 kg; Custom packaging on request
Water content
K.F. <=0.20% w/w
Physical form
white to light yellow powder

用途情報

  • RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
  • 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
  • N2-dmf base protection: the dimethylformamidine group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What are the four functional groups on this RNA amidite?

A 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite (the reactive coupling group), an acid-labile 5'-O-DMT group (removed each cycle), a 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and an N2-dimethylformamidine (dmf) base protection (removed at final deprotection).

How does it differ from the 2'-O-TBDMS-rG(iBu) phosphoramidite?

Both are 2'-O-TBDMS guanosine RNA amidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.

類似製品

2',3',5'-Tri-O-acetyl-D-adenosine

CAS番号
7387-57-7
分子式
C16H19N5O7
分子量
393.35

L-Threoninol

CAS番号
3228-51-1
分子式
C4H11NO2
分子量
105.14

Tetrabenzyl pyrophosphate

CAS番号
990-91-0
分子式
C28H28O7P2
分子量
538.47

2'-Deoxyadenosine Monohydrate

CAS番号
16373-93-6
分子式
C10H15N5O4
分子量
269.26

材料選択の支援が必要ですか？

CHEMOSは製品適合性、目標構造、ルート上の課題、分析要件、プロジェクト別供給ニーズを確認します。

プロジェクト支援を見る