Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-Guanosine (dmf) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65021CAS番号128219-77-2純度>=98.5% (HPLC)

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製品概要

クイックビュー

製品ファミリー: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C44H55N8O8P
分子量: 854.9
純度: >=98.5% (HPLC)

2'-OMe-Guanosine (dmf) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-dimethylformamidine-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65021 is identified by CAS 128219-77-2, molecular formula C44H55N8O8P, molecular weight 854.9, PubChem CID 135742510, and InChIKey ZGDBCILCTOHNAB-OWKWDSSOSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

別名

DMT-2'-OMe-rG(dmf) phosphoramidite; 5'-O-DMT-N2-dmf-2'-O-methylguanosine 3'-CE phosphoramidite

同定情報と仕様

Catalog No.	CH65021
CAS No.	128219-77-2
Molecular Formula	C44H55N8O8P
Molecular Weight	854.9
PubChem CID	135742510
InChIKey	ZGDBCILCTOHNAB-OWKWDSSOSA-N
Purity	>=98.5% (HPLC)

保管条件

-20 C

技術属性

Monomer class: 2'-O-methyl RNA CE phosphoramidite
Nucleobase: Guanine
Sugar modification: 2'-O-Methyl
Base protection: N2-Dimethylaminomethylene (dmf)
Grade: N (Normal)
Packaging: 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
Water content: <=0.5%
Physical form: white to off-white powder
Stability: >=4 years

用途情報

2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.

公開情報源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem化合物 135742510
PubChem · CID 135742510

よくある質問

What does this 2'-OMe amidite do in RNA synthesis?

Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection. The product provides a 2'-O-methylguanosine phosphoramidite identity.

How does it differ from the 2'-OMe-G(iBu) phosphoramidite?

Both are 2'-O-methyl guanosine amidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.