CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-5-Methyluridine CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65025CAS番号153631-20-0純度>99% (NMR)
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製品概要

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製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C41H51N4O9P
分子量
774.8
純度
>99% (NMR)

2'-OMe-5-Methyluridine CE Phosphoramidite is the 2'-O-methyl 5-methyluridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-methyl-5-methyluridine (5-methyluracil base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65025 is identified by CAS 153631-20-0, molecular formula C41H51N4O9P, molecular weight 774.8, PubChem CID 10327870, and InChIKey GMJRPRHRYILEOK-VNZAIEIISA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

別名

DMT-2'-OMe-5Me-rU phosphoramidite; 5'-O-DMT-2'-O-methyl-5-methyluridine 3'-CE phosphoramidite

同定情報と仕様

Catalog No.CH65025
CAS No.153631-20-0
Molecular FormulaC41H51N4O9P
Molecular Weight774.8
PubChem CID10327870
InChIKeyGMJRPRHRYILEOK-VNZAIEIISA-N
Purity>99% (NMR)

保管条件

-20 C

技術属性

Monomer class
2'-O-methyl RNA CE phosphoramidite
Nucleobase
5-Methyluracil
Sugar modification
2'-O-Methyl
Base protection
Unprotected
Packaging
100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 100 g; Custom packaging on request
Physical form
solid
Stability
>=4 years

用途情報

  • 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
  • 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
  • No base protection needed: the 5-methyluracil base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

Why does this 2'-OMe amidite have no base protecting group?

Its base is 5-methyluracil, which has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.

How does it relate to 5'-O-DMT-5-methyl-2'-O-methyluridine?

It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.

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