Oligonucleotide Synthesis, RNA Raw Materials & Modification

5-aa-2'-dUTP (Aminoallyl-dUTP)

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66011CAS番号936327-10-5純度>=98%

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製品概要

クイックビュー

製品ファミリー: Oligonucleotide Synthesis, RNA Raw Materials & Modification
純度: >=98%

5-aa-2'-dUTP is 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate, commonly described as aminoallyl-dUTP. It is a modified deoxynucleotide triphosphate that carries a primary amine on a 5-aminoallyl linker at the C5 position of uracil. Key identifiers for this catalog entry are CHEMOS CH66011 and CAS 936327-10-5.

In enzymatic DNA labeling workflows, aminoallyl-dUTP can be incorporated by DNA polymerases during template-directed DNA synthesis. The introduced primary amine can then react with amine-reactive reporters such as NHS-ester dyes, allowing post-synthetic labeling of DNA products. This page keeps the identity boundary to the supplier catalog name, CAS number, and literature-supported aminoallyl-dUTP use context.

別名

aminoallyl-dUTP; 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate; AA-dUTP

同定情報と仕様

Catalog No.	CH66011
Product Name	5-aa-2'-dUTP (Aminoallyl-dUTP)
CAS No.	936327-10-5
Product Type	Amine-modified deoxynucleotide triphosphate
Labeling Handle	5-aminoallyl primary amine
Purity	>=98%

保管条件

freeze below -15 C; minimize light exposure

技術属性

Nucleotide class: dNTP
Parent nucleotide: dUTP
Base modification: 5-(3-Aminoallyl)
Reactive handle: Primary amine
Packaging: 1 mg; 5 mg; 25 mg; 50 mg; 100 mg; 5 x 1 mg; Custom packaging on request
Solution concentration: 4 mM
Physical form: colorless liquid
Stability: 6 months upon receiving

用途情報

Amine handle in DNA: used as an aminoallyl-modified dUTP to introduce a primary amine into enzymatically synthesized DNA.
Post-synthetic labeling: the aminoallyl handle can react with amine-reactive reporters such as NHS-ester dyes.
Probe preparation: relevant to DNA labeling workflows such as PCR, reverse-transcription labeling, and microarray probe preparation.

公開情報源

Aminoallyl-dUTP sodium salt, catalog A0410
Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context

よくある質問

What is 5-aa-2'-dUTP used for?

5-aa-2'-dUTP is an aminoallyl-modified dUTP used to introduce a primary amine into enzymatically synthesized DNA for post-synthetic labeling.

Why use an aminoallyl handle?

The aminoallyl group provides a small primary amine handle that can react with amine-reactive reporters such as NHS-ester dyes after nucleotide incorporation.