Oligonucleotide Synthesis, RNA Raw Materials & Modification

7-ap-7-Deaza-2'-dATP

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66016CAS番号587848-72-4純度>98.00%

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製品概要

クイックビュー

製品ファミリー: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C14H20N5O12P3
分子量: 543.26
純度: >98.00%

7-ap-7-Deaza-2'-dATP is 7-(3-aminopropargyl)-7-deaza-2'-deoxyadenosine-5'-triphosphate: a 7-deazaadenine dATP analogue (the purine N7 is replaced by C7) carrying a primary amine on a propargyl (alkyne) linker at that C7 position. Key identifiers include CAS 587848-72-4, molecular formula C14H20N5O12P3, and molecular weight 543.26 (PubChem CID 101377226).

DNA polymerases incorporate deoxynucleoside triphosphate substrates during template-directed DNA synthesis. This amine-modified 7-deaza-dATP places a primary amine into the DNA — presented in the major groove by the 7-deaza scaffold — and that aliphatic amine then reacts specifically with amine-reactive reporters such as NHS-ester dyes, so the DNA can be labeled after synthesis.

別名

7-(3-aminopropargyl)-7-deaza-2'-deoxyadenosine-5'-triphosphate; 7-deaza-7-propargylamino-dATP

同定情報と仕様

Catalog No.	CH66016
CAS No.	587848-72-4
Molecular Formula	C14H20N5O12P3
Molecular Weight	543.26
PubChem CID	101377226
InChIKey	PCAJRNFTCPOUJA-HBNTYKKESA-N
Purity	>98.00%

保管条件

-20 C

技術属性

Nucleotide class: dNTP analogue
Nucleobase: 7-Deazaadenine
Base modification: 7-(3-Aminopropargyl)
Reactive handle: Primary amine
Packaging: 1 mg; 50 mg; 100 mg; 250 mg; 10 µL; 50 µL; 100 µL; 10 x 100 uL; 5 x 100 uL; Custom packaging on request
Solution concentration: 100 mM
Physical form: solid; colorless to white
Stability: 12 months after delivery; cumulative ambient exposure up to 1 week possible

用途情報

Amine label handle at adenine: incorporated by DNA polymerase during enzymatic DNA synthesis, placing a primary amine (on a C7 propargyl linker) at adenine positions.
7-deaza scaffold: the purine N7 is replaced by C7, positioning the label in the major groove.
Post-synthetic dye labeling: the amine reacts with amine-reactive reporters (e.g. NHS-ester dyes) to label the DNA.

公開情報源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem化合物 101377226
PubChem · CID 101377226

よくある質問

What is 7-ap-7-Deaza-2'-dATP used for?

It is a 7-deaza-dATP analogue with a primary amine on a C7 propargyl linker. DNA polymerases incorporate it during enzymatic DNA synthesis at adenine positions, placing an amine in the DNA; the amine then reacts with amine-reactive dyes (e.g. NHS esters) to label the product.

What does '7-deaza' mean?

In a 7-deaza purine, the nitrogen at position 7 of the purine ring is replaced by a carbon. This provides an attachment point (C7) for the label in the major groove and is a common scaffold for labeled purine nucleotides.