CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

5-ap-ddCTP (Amino-dideoxy-CTP)

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66018CAS番号114748-56-0純度99.0%
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製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C12H19N4O12P3
分子量
504.22
純度
99.0%

5-ap-ddCTP is 5-(3-aminopropargyl)-2',3'-dideoxycytidine-5'-triphosphate: a 2',3'-dideoxy CTP (a chain terminator) carrying a primary amine on a propargyl (alkyne) linker at the C5 of cytosine. Key identifiers include CAS 114748-56-0, molecular formula C12H19N4O12P3, and molecular weight 504.22 (PubChem CID 21779042).

2',3'-dideoxynucleoside triphosphates act as specific chain-terminating inhibitors of DNA polymerase — the basis of Sanger dideoxy sequencing: once one is incorporated, the missing 3'-OH stops further extension. The C5 primary amine is a labelling handle for attaching a dye, so this reagent serves as a building block for labeled dye-terminators.

別名

5-(3-aminopropargyl)-2',3'-dideoxycytidine-5'-triphosphate; amino-ddCTP

同定情報と仕様

Catalog No.CH66018
CAS No.114748-56-0
Molecular FormulaC12H19N4O12P3
Molecular Weight504.22
PubChem CID21779042
InChIKeyUZTVDBNPCNJIGN-VHSXEESVSA-N
Purity99.0%

保管条件

below -15 C; minimize light exposure

技術属性

Nucleotide class
Chain-terminating ddNTP
Nucleobase
Cytosine
Base modification
5-(3-Aminopropargyl)
Reactive handle
Primary amine
Packaging
25 mg; 50 mg; 100 mg; 250 mg; Custom packaging on request
Solution concentration
25 mM
Physical form
liquid; colorless; water solvent
Stability
6 months upon receiving

用途情報

  • Chain terminator: a 2',3'-dideoxy-CTP that stops DNA-polymerase extension when incorporated (the basis of Sanger sequencing).
  • Amine label handle: the C5 aminopropargyl group lets a dye be attached, giving a labeled terminator.
  • Dye-terminator building block: used to prepare fluorescent ddNTP terminators for sequencing.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What is 5-ap-ddCTP used for?

It is a 2',3'-dideoxy-CTP chain terminator with a C5 aminopropargyl amine handle. As a dideoxy nucleotide it stops DNA-polymerase extension (the basis of Sanger sequencing); the amine allows a dye to be attached, making it a building block for labeled dye-terminators.

Why does the dideoxy nucleotide terminate synthesis?

A 2',3'-dideoxynucleotide lacks the 3'-hydroxyl needed to form the next internucleotide bond. Once a polymerase incorporates it, extension stops — the principle behind dideoxy (Sanger) sequencing.

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