CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

5-ap-ddUTP (Amino-dideoxy-UTP)

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66019CAS番号114748-59-3純度98%
製品画像はありません

製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C12H18N3O13P3
分子量
505.20
純度
98%

5-ap-ddUTP is 5-(3-aminopropargyl)-2',3'-dideoxyuridine-5'-triphosphate: a 2',3'-dideoxy UTP (a chain terminator) carrying a primary amine on a propargyl (alkyne) linker at the C5 of uracil. Key identifiers include CAS 114748-59-3, molecular formula C12H18N3O13P3, and molecular weight 505.20 (PubChem CID 11071202).

2',3'-dideoxynucleoside triphosphates act as specific chain-terminating inhibitors of DNA polymerase — the basis of Sanger dideoxy sequencing: once one is incorporated, the missing 3'-OH stops further extension. The C5 primary amine is a labelling handle for attaching a dye, so this reagent serves as a building block for labeled dye-terminators.

別名

5-(3-aminopropargyl)-2',3'-dideoxyuridine-5'-triphosphate; amino-ddUTP

同定情報と仕様

Catalog No.CH66019
CAS No.114748-59-3
Molecular FormulaC12H18N3O13P3
Molecular Weight505.20
PubChem CID11071202
InChIKeyHOYCULDIPQRPFI-VHSXEESVSA-N
Purity98%

保管条件

-20 C

技術属性

Nucleotide class
Chain-terminating ddNTP
Nucleobase
Uracil
Base modification
5-(3-Aminopropargyl)
Reactive handle
Primary amine
Packaging
1 mg; 25 mg; 50 mg; 100 mg; 250 mg; Custom packaging on request
Solution concentration
100 mM
Physical form
solid; white to off-white
Stability
12 months after delivery; cumulative ambient exposure up to 1 week possible

用途情報

  • Chain terminator: a 2',3'-dideoxy-UTP that stops DNA-polymerase extension when incorporated (the basis of Sanger sequencing).
  • Amine label handle: the C5 aminopropargyl group lets a dye be attached, giving a labeled terminator.
  • Dye-terminator building block: used to prepare fluorescent ddNTP terminators for sequencing.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What is 5-ap-ddUTP used for?

It is a 2',3'-dideoxy-UTP chain terminator with a C5 aminopropargyl amine handle. As a dideoxy nucleotide it stops DNA-polymerase extension (the basis of Sanger sequencing); the amine allows a dye to be attached, making it a building block for labeled dye-terminators.

Why does the dideoxy nucleotide terminate synthesis?

A 2',3'-dideoxynucleotide lacks the 3'-hydroxyl needed to form the next internucleotide bond. Once a polymerase incorporates it, extension stops — the principle behind dideoxy (Sanger) sequencing.

類似製品

2',3',5'-Tri-O-acetyl-D-adenosine

CAS番号
7387-57-7
分子式
C16H19N5O7
分子量
393.35

L-Threoninol

CAS番号
3228-51-1
分子式
C4H11NO2
分子量
105.14

Tetrabenzyl pyrophosphate

CAS番号
990-91-0
分子式
C28H28O7P2
分子量
538.47

2'-Deoxyadenosine Monohydrate

CAS番号
16373-93-6
分子式
C10H15N5O4
分子量
269.26

材料選択の支援が必要ですか？

CHEMOSは製品適合性、目標構造、ルート上の課題、分析要件、プロジェクト別供給ニーズを確認します。

プロジェクト支援を見る