CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

7-ap-7-Deaza-ddGTP

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66021CAS番号114748-61-7純度>=93% (HPLC)
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製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C14H20N5O12P3
分子量
543.26
純度
>=93% (HPLC)

7-ap-7-Deaza-ddGTP is 7-(3-aminopropargyl)-7-deaza-2',3'-dideoxyguanosine-5'-triphosphate: a 7-deaza (purine N7 replaced by C7) 2',3'-dideoxy GTP chain terminator carrying a primary amine on a propargyl (alkyne) linker at C7. Key identifiers include CAS 114748-61-7, molecular formula C14H20N5O12P3, and molecular weight 543.26 (PubChem CID 135577908).

2',3'-dideoxynucleoside triphosphates act as specific chain-terminating inhibitors of DNA polymerase — the basis of Sanger dideoxy sequencing: once one is incorporated, the missing 3'-OH stops further extension. The C7 primary amine is a labelling handle for attaching a dye, presented in the major groove by the 7-deaza scaffold, so this reagent serves as a building block for labeled dye-terminators.

別名

7-(3-aminopropargyl)-7-deaza-2',3'-dideoxyguanosine-5'-triphosphate; amino-7-deaza-ddGTP

同定情報と仕様

Catalog No.CH66021
CAS No.114748-61-7
Molecular FormulaC14H20N5O12P3
Molecular Weight543.26
PubChem CID135577908
InChIKeyLJJGDWYGTRBWBZ-VHSXEESVSA-N
Purity>=93% (HPLC)

保管条件

-20 C

技術属性

Nucleotide class
Chain-terminating ddNTP
Nucleobase
7-Deazaguanine
Base modification
7-(3-Aminopropargyl)
Reactive handle
Primary amine
Packaging
1 mg; 50 mg; 100 mg; 500 mg; Custom packaging on request
Physical form
solid; white to off-white
Stability
12 months after delivery; cumulative ambient exposure up to 1 week possible

用途情報

  • Chain terminator: a 7-deaza 2',3'-dideoxy-GTP that stops DNA-polymerase extension when incorporated (the basis of Sanger sequencing).
  • Amine label handle in the major groove: the C7 aminopropargyl group (on the 7-deaza scaffold) lets a dye be attached, giving a labeled terminator.
  • Dye-terminator building block: used to prepare fluorescent ddNTP terminators for sequencing.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What is 7-ap-7-Deaza-ddGTP used for?

It is a 7-deaza 2',3'-dideoxy-GTP chain terminator with a C7 aminopropargyl amine handle. As a dideoxy nucleotide it stops DNA-polymerase extension (the basis of Sanger sequencing); the amine allows a dye to be attached, making it a building block for labeled dye-terminators.

Why the 7-deaza modification on a dideoxy terminator?

Replacing the purine N7 with C7 provides an attachment point in the major groove for the label and avoids a hydrogen-bonding nitrogen there. Combined with the missing 3'-OH (which terminates synthesis), it gives a well-behaved labeled purine terminator.

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