Oligonucleotide Synthesis, RNA Raw Materials & Modification

3'-O-Ac-2'-dTTP

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66033CAS番号90290-82-7純度>95% (HPLC)

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製品概要

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製品ファミリー: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C12H19N2O15P3
分子量: 524.20
純度: >95% (HPLC)

3'-O-Ac-2'-dTTP is 3'-O-acetyl-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. Key identifiers include CAS 90290-82-7, molecular formula C12H19N2O15P3, and molecular weight 524.20 (PubChem CID 168301121).

3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-acetyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.

別名

3'-O-acetyl-dTTP; 3'-O-(acetyl)-thymidine-5'-triphosphate

同定情報と仕様

Catalog No.	CH66033
CAS No.	90290-82-7
Molecular Formula	C12H19N2O15P3
Molecular Weight	524.20
PubChem CID	168301121
InChIKey	PEHYLTBXIMDUCN-IVZWLZJFSA-N
Purity	>95% (HPLC)

保管条件

-70 C

技術属性

Nucleotide class: Reversible-terminator dNTP
Parent nucleotide: dTTP
3'-O blocking group: Acetyl
Deblocking outcome: Regenerates the 3'-OH
Packaging: 10 mg; 50 mg; 100 mg; 250 mg; 5 umol (~2.6 mg) in 500 uL vial; Custom packaging on request
Solution concentration: 10 mM
Physical form: 10 mM aqueous sodium-salt solution
Stability: slowly decomposes at ambient temperature; thaw only before use and avoid repeated freeze/thaw cycles

用途情報

Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
Cap removal reinitiates: removing the 3'-O-acetyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
Thymine building block: supplies the thymine reversible terminator, completing the four-nucleotide (A/C/G/T) 3'-O-acetyl SBS set.

公開情報源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem化合物 168301121
PubChem · CID 168301121

よくある質問

What is a 3'-O-acetyl reversible terminator?

It is a dNTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the acetyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.

Which base does this terminator add?

It is the thymine (T) reversible terminator — 3'-O-acetyl-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-acetyl terminators it forms a four-nucleotide set for sequencing-by-synthesis.