CHEMOS
Oligonucleotide Synthesis, RNA Raw Materials & Modification

3'-O-azidomethyl-dTTP

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH66034CAS番号1048021-85-7純度99.98%
製品画像はありません

製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C11H18N5O14P3
分子量
537.21
純度
99.98%

3'-O-azidomethyl-dTTP is 3'-O-azidomethyl-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with an azidomethyl (-CH2N3) group. Key identifiers include CAS 1048021-85-7, molecular formula C11H18N5O14P3, and molecular weight 537.21 (PubChem CID 121487868).

This is the reversible-terminator chemistry underlying cyclic sequencing-by-synthesis: 3'-O-azidomethyl-dNTPs are still recognized by DNA polymerase as substrates and act as reversible terminators, so incorporation adds one base and stops extension. After each incorporation and readout, the azidomethyl cap is removed with a water-soluble phosphine (TCEP) to regenerate the 3'-OH so the polymerase reinitiates.

別名

3'-O-azidomethyl-dTTP; 3'-O-(azidomethyl)-thymidine-5'-triphosphate

同定情報と仕様

Catalog No.CH66034
CAS No.1048021-85-7
Molecular FormulaC11H18N5O14P3
Molecular Weight537.21
PubChem CID121487868
InChIKeyNLCMYGVMMMRQPV-DJLDLDEBSA-N
Purity99.98%

保管条件

solution at -20 C

技術属性

Nucleotide class
Reversible-terminator dNTP
Parent nucleotide
dTTP
3'-O blocking group
Azidomethyl
Deblocking reagent
TCEP
Packaging
50 mg; 100 mg; 250 mg; 100 µL; 200 µL; 1 mL; 5 mL; 10 mL; Custom packaging on request
Solution concentration
100 mM
Physical form
liquid solution; colorless to light yellow
Stability
2 years as solution at -20 C

用途情報

  • Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
  • TCEP-cleavable cap: the 3'-O-azidomethyl cap is removed with tris(2-carboxyethyl)phosphine (TCEP) to regenerate the 3'-OH so synthesis continues.
  • Sequencing-by-synthesis chemistry: the azidomethyl reversible terminator is the chemistry behind cyclic four-colour SBS.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

What is a 3'-O-azidomethyl reversible terminator?

It is a dNTP whose 3'-hydroxyl is capped with an azidomethyl group. DNA polymerase still incorporates it, but the cap stops further extension after one base. The azidomethyl cap is removed with a water-soluble phosphine (TCEP) to regenerate the 3'-OH so synthesis can continue — the chemistry behind cyclic reversible-terminator sequencing-by-synthesis.

How is the cap removed between cycles?

The azidomethyl group is cleaved with tris(2-carboxyethyl)phosphine (TCEP), a water-soluble reducing agent that is compatible with DNA. This regenerates the free 3'-OH so the next base can be added.

類似製品

2',3',5'-Tri-O-acetyl-D-adenosine

CAS番号
7387-57-7
分子式
C16H19N5O7
分子量
393.35

L-Threoninol

CAS番号
3228-51-1
分子式
C4H11NO2
分子量
105.14

Tetrabenzyl pyrophosphate

CAS番号
990-91-0
分子式
C28H28O7P2
分子量
538.47

2'-Deoxyadenosine Monohydrate

CAS番号
16373-93-6
分子式
C10H15N5O4
分子量
269.26

材料選択の支援が必要ですか？

CHEMOSは製品適合性、目標構造、ルート上の課題、分析要件、プロジェクト別供給ニーズを確認します。

プロジェクト支援を見る