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Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-O-TBDMS-A (Bz) 3'-PS Thiophosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

製品番号CH65080CAS番号1374755-65-3純度>=95%
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製品概要

クイックビュー

製品ファミリー
Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式
C57H65N6O8PS2Si
分子量
1085.4
純度
>=95%

2'-O-TBDMS-A (Bz) 3'-PS Thiophosphoramidite is a phosphorothioate-linkage RNA building block: a 5'-O-(4,4'-dimethoxytrityl)-N6-benzoyl-2'-O-(tert-butyldimethylsilyl)adenosine 3'-thiophosphoramidite. It is identified by CAS 1374755-65-3, molecular formula C57H65N6O8PS2Si, molecular weight 1085.4, PubChem CID 102308172, and InChIKey ALJIYXCITZABFP-WOMFGPHHSA-N.

As a thiophosphoramidite it provides the sulfur-bearing phosphorus chemistry used for phosphorothioate (PS) linkage synthesis. The cited phosphorothioate literature discusses PS linkages in relation to nuclease resistance. The 2'-O-TBDMS group is the silyl protecting group for the 2'-hydroxyl in RNA synthesis; the 5'-DMT is removed each cycle and the N6-benzoyl base-protecting group is removed during final deprotection.

別名

2-OTBS A(Bz) ThioPhosphamidite; 5'-O-DMT-N6-Bz-2'-O-TBDMS-adenosine 3'-thiophosphoramidite

同定情報と仕様

Catalog No.CH65080
CAS No.1374755-65-3
Molecular FormulaC57H65N6O8PS2Si
Molecular Weight1085.4
PubChem CID102308172
InChIKeyALJIYXCITZABFP-WOMFGPHHSA-N
Purity>=95%

保管条件

-20 C

技術属性

Monomer class
RNA thiophosphoramidite
Nucleobase
Adenine
2'-O protection
TBDMS
Linkage role
Phosphorothioate linkage precursor
Packaging
1 g; Custom packaging on request

用途情報

  • Phosphorothioate (PS) linkage: the thiophosphoramidite provides sulfur-bearing phosphorus chemistry for PS linkage synthesis.
  • PS literature context: the cited phosphorothioate literature discusses PS linkages in relation to nuclease resistance.
  • 2'-O-TBDMS RNA protection: the tert-butyldimethylsilyl group protects the 2'-hydroxyl during solid-phase RNA synthesis.
  • Protection scheme: the 5'-DMT is removed each cycle and the N6-benzoyl base-protecting group is removed during final deprotection.

関連技術資料

オリゴヌクレオチド修飾

ASO、siRNA、guide RNA、aptamer、研究用オリゴの糖、塩基、骨格、末端、コンジュゲーション修飾。

オリゴヌクレオチド合成

修飾モノマー、担体、硫化試薬、キャップ 類似体、関連するオリゴ合成入力物の支援。

公開情報源

よくある質問

How does this differ from the standard 2'-O-TBDMS-rA(Bz) amidite?

It is a thiophosphoramidite rather than a standard phosphoramidite: the phosphorus chemistry is used for phosphorothioate (PS) linkage synthesis, whereas the standard amidite is used for phosphodiester linkage synthesis. Both carry 5'-DMT, 2'-O-TBDMS, and N6-benzoyl protection.

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